Driven by a passion for bioinformatics and genomic technologies, I quickly mastered new materials at Al-Quds Bard College and the Genome Centre, demonstrating exceptional adaptability and problem-solving skills. My ability to lead and collaborate effectively, coupled with my technical proficiency, has consistently improved project outcomes, positioning me as a valuable asset to any team.
-Graduation Project: Examining the Consequences of H19 RNA Knockdown on the Expression Levels of Apoptotic Genes under Hypoxic Conditions.
-Description: Investigated the role of H19 long non-coding RNA in regulating apoptotic gene expression in cancer cells under hypoxic conditions. Utilized shRNA constructs to knock down H19 expression in HT-29 and MDA-231 cell lines, representing colorectal adenocarcinoma and breast cancer. Aimed to uncover molecular mechanisms underlying cancer cell survival in low oxygen environments, providing insights for targeted therapeutic approaches.
_Technologies Used:
- Transfection of shRNA Plasmids to Competent Bacteria
- Molecular Biology Techniques (e.g., RNA interference using shRNA constructs)
- Cell Culture (maintaining HT-29 and MDA-231 cancer cell lines under normoxic and hypoxic conditions)
- Gene Expression Analysis (polymerase chain reaction (PCR), gel electrophoresis)
- Bioinformatics Tools (analyzing gene expression data, identifying interactions between H19 RNA and apoptotic genes
Project Support
Completed a novel study on the relationship between survivin and the knockdown of the H19 gene under hypoxic conditions as part of my final project for my Bachelor's degree in Molecular Genetics. This research, a milestone in molecular genetics, enhanced my research skills and provided valuable insights into cancer development by uncovering the survivin and H19 gene relationship.
- Dr. Mahmoud Khalid
Assistant Dean, Head of Science Division
Biology Lecturer
Al-Quds Bard College for Arts and Science, Al-Quds University
Palestine, East Jerusalem
Email: mkhalid@staff.alquds.edu
- Dr. Imad Matouk, PhD
Associate Professor, Head of Molecular Genetics Program
Biology Department, Al-Quds University
Email: imatouk@staff.alquds.edu