Muhammad Altaf Khan PhD
West Haven,USA
Summary
Accomplished Biotechnologist with extensive expertise in RNA and DNA extraction, PCR, and advanced sequencing techniques, including Sanger and next-generation sequencing. Proficient in gene cloning, recombinant gene expression in E. coli and yeast, and a variety of genotyping techniques such as SSR, ISSR, RAPD, SCAR, AFLP, SRAP, and SNP. Skilled in protein purification using AKTA-FPLC and HPLC systems, with experience in folding recombinant proteins and conducting 2D-PAGE and DIGE analyses. Adept at using bioinformatics tools for sequencing analysis and functional annotation. Career goals include advancing research in molecular genetics through innovative applications of cutting-edge technologies.
Overview
23
23
years of professional experience
6013
6013
years of post-secondary education
Work history
Research Assistant Professor
King Saud University
Riyadh, Saudi Arabia
01.2014 - 09.2025
- Conducted sequencing experiments and analysed results from various genotyping techniques.
- Established proteomic laboratory, providing training in advanced proteomic techniques.
- Taught molecular biology methods to graduate and postgraduate students.
- Designed research projects for graduate and postgraduate students, ensuring alignment with academic objectives.
- Authored research articles and project reports based on experimental findings.
- Mentored MS and PhD students throughout experiments and dissertation writing process.
Experimental Officer
School of Biological Sciences, University of the Punjab
Lahore
01.2009 - 01.2010
- Designed and constructed a vector for high-level gene expression.
- Cloned and expressed the caprine and buffalo growth hormone gene.
- Increased recombinant expression by introducing silent mutations at the 5'-end.
- Performed the refolding and purification of recombinant proteins.
- Performed bioassays on the purified proteins.
- Provided technical services for DNA sequencing and medium-scale fermentation experiments.
Research Officer (PhD Student)
School of Biological Sciences, University of the Punjab
Lahore
01.2003 - 01.2009
- Designed and constructed a vector for high-level gene expression.
- Cloned and expressed the caprine and buffalo growth hormone gene.
- Increased recombinant expression by introducing silent mutations at the 5'-end.
- Performed the refolding and purification of recombinant proteins.
- Performed bioassays on the purified proteins.
- Provided technical services for DNA sequencing and medium-scale fermentation experiments.
Education
Ph.D - Biochemistry and Biotechnology
The University of the Punjab
Lahore, PakistanM.Sc - Biochemistry
The University of the Punjab
Lahore-PakistanB.Sc. - Zoology, Botany & Chemistry
The University of the Punjab
Lahore-pakistanSkills
- Biochemistry
- Molecular Biology
- Proteomics
- DNA Sequencing
Skills
- RNA and DNA Extraction
- PCR
- RT-PCR
- Quantitative Real-Time PCR (qRT-PCR)
- Gene Cloning
- DNA Sequencing (Sanger and Next generation sequencing)
- DNA Recombinant Techniques
- Recombinant Gene Expression (in E coli and Yeast)
- Genotyping Techniques (SSR, ISSR, RAPD, SCAR, AFLP, SRAP, SNP)
- Protein Electrophoresis
- Protein Purification techniques (AKTA-FPLC, HPLC)
- Folding of recombinant proteins
- 2D-PAGE
- DIGE (Differential Gel Electrophoresis)
- Western blot
- Mass Spectrometry (Maldi Tof Tof)
- Working experience with Nb2 lymphoma cell lines
- Working experience with chickpea explants
- Sequencing analysis (Sanger and Next Generation Sequencing)
- General use of bioinformatics tools (ClustalW, BLAST, codon code aligner, DNA Baser Assembler)
- Functional annotation
- Genemapper
- Phylogenetic analysis software (NTsys)
- Protein Structure Prediction
- Homology modeling tools
Languages
English
Advanced
Urdu
Native
Arabic
Elementary
Punjabi
Native
Accomplishments
Ø Travel award 21th IUBMB and 12th FAOBMB International Congress of Biochemistry and Molecular Biology and Young Scientist Program, Shanghai, China, July 30-August 7, 2009
Ø Ph.D. Fellowship in the School of Biological Sciences, University of the Punjab, Lahore, 2003
Professional Trainings
- DNA Sequencing Training, Beckman Coulter, High Wycombe, London, 2005
- Next generation Sequencing Training, Beijing Genome Institute (BGI), Shenzhen, China, 2017
Research Publications
47 publications.
Google Scholar: https://scholar.google.com/citations?user=-Hx5N-0AAAAJ&hl=en
Timeline
Research Assistant Professor
King Saud University
01.2014 - 09.2025
Experimental Officer
School of Biological Sciences, University of the Punjab
01.2009 - 01.2010
Research Officer (PhD Student)
School of Biological Sciences, University of the Punjab
01.2003 - 01.2009
Ph.D - Biochemistry and Biotechnology
The University of the Punjab
M.Sc - Biochemistry
The University of the Punjab
B.Sc. - Zoology, Botany & Chemistry
The University of the Punjab
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